İstanbul Galata Üniversitesi Kurumsal Akademik Arşivi
DSpace@Galata, İstanbul Galata Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Post-transcriptional knockdown of BRCC3 via siRNA-loaded niosomes modulates autophagy and endoplasmic reticulum stress in rotenone-induced Parkinson's Disease model
(2026) Gürel, Çevik; Tut, Ezgi; Kuşçu, Gökçe Ceren; Buhur, Aylin; Germiyan, Özgün Selim; Leila Sabour-Takanlou; Maryam Sabour-Takanlou; Güler, Cem; Yavaşoğlu, Nefise Ülkü Karabay; Oltulu, Fatih; Yavaşoğlu, Altuğ
Parkinson's Disease (PD) is a prevalent neurodegenerative disorder. Recent studies implicate BRCA1-/BRCA2-containing complex 3 (BRCC3) in PD-related mechanisms such as ubiquitin-proteasome system dysfunction. This study aimed to evaluate the therapeutic potential of BRCC3 silencing via systemically administered siRNA-loaded niosomes in a rotenone-induced rat model of PD. Niosomes were synthesised by thin-film hydration, and three BRCC3-targeted siRNA sequences were tested in primary midbrain dopaminergic neurons. The most effective sequence, identified by Real-Time Quantitative PCR (RT-qPCR) and immunofluorescence, was used for in vivo studies. The PD model was induced in adult male rats (n = 24/group) by subcutaneous rotenone administration (2 mg/kg/day) for 35 days. In the in vivo phase of the study, behavioural, biochemical, in vivo imaging (IVIS), histological, and RT-qPCR analyses were performed. IVIS analysis confirmed brain accumulation of niosome-siRNA complexes within 3-5 h. Complementary analyses demonstrated that siRNA treatment significantly enhanced locomotor performance, restored redox homeostasis and dopamine levels, attenuated neuronal loss, upregulated autophagy-related proteins (↑LC3-II, ↑Beclin), suppressed endoplasmic reticulum stress markers (↓GRP78/Bip, ↓CHOP), elevated tyrosine hydroxylase expression, and reduced α-synuclein accumulation. In conclusion, siRNA-mediated suppression of BRCC3 via siRNA-loaded niosomes provides neuroprotection by modulating autophagy, ER stress, and antioxidant pathways, supporting BRCC3 as a promising therapeutic target for PD.
Aesthetic efficacy of resin infiltration on post-orthodontic white spot lesions using different conditioning methods: 24-month randomized control follow-up
(Allen Press Inc., 2026) Karaman, Emel; Ünsal, Kübra Alan; Öz, Aslıhan Zeynep; Öz, Abdullah Alper; Ercin, Özlem
Objectives: To assess color and fluorescence changes in post-orthodontic white spot lesions (WSLs) using different surface conditioning methods from prior to resin infiltration to a 24-month follow-up. Materials and Methods: Thirty patients, each with a minimum of four WSLs with ICDAS (International Caries Detection and Assessment System) II code 1 and 2 after bracket debonding were included. After baseline (T0), the lesions were randomly divided into four groups: regular brushing, 15% HCl (hydrogen chloride) gel, 37% H3PO4(phosphoric acid) gel, and Er:YAG (erbium-doped yttrium aluminium garnet) laser. Icon resin infiltration was applied. In the treatment groups, color and fluorescence were reexamined just after the resin infiltration treatment (T1), and after 6 (T2) and 24 months (T3). Results: There were no significant fluorescence or color changes between the treatment groups for the different time intervals. The control group showed a significantly lower color change from T0 to T2 and T0 to T3 than all the treatment groups whereas, at T2–T3, the control group showed the greatest improvement. Conclusions: Resin infiltration caused greater esthetic improvement and more revival of the lost fluorescence than daily brushing. HCl gel, H3PO4gel, and Er:YAG laser are adequate conditioning methods for resin infiltration of post-orthodontic WSLs with ICDAS II code 1 and 2. (Angle Orthod. 2026;96:311–317.). © 2026, Allen Press Inc. All rights reserved.
Validity and Reliability of the Turkish Version of the Diabetes Education Questionnaire in People with Diabetes
(AVES, 2026) Şafak, Yiğit; Akıncı, Buket; Ekşi, Büşra Ülker; İdiz, Cemile; Şahin, Hümeyra Rekalı; Çevikdizci, Melike; Ghisi, Gabriela Lima de Melo; Satman, İlhan
Objective: Patient education is a key component of diabetes management and chronic disease care, facilitating behavioral changes and supporting effective self-management. Reliable instruments for assessing patients’ diabetes knowledge are essential for identifying educational needs and designing evidence-based strategies. The purpose of this study was to examine the reliability and validity of the Turkish adaptation of the Diabetes Education Questionnaire (DATE-Q) in evaluating the diabetes knowledge of individuals with diabetes. Methods: This methodological study was conducted with 200 people with type 1 (T1DM) or type 2 diabetes mellitus (T2DM). The study involved translation, back-translation, and cross-cultural adaptation of the DATE-Q. The Turkish version of the Revised Diabetes Knowledge Test (DKT2) was used for validation. Criterion validity was assessed using Spearman's correlation between DATE-Q and DKT2. Cronbach’s alpha and intraclass correlation coefficient (ICC) values were used to determine the reliability. Results: Participants had a mean age of 55.9 ± 12.5 years, 63% were female, and 82.5% had T2DM. The Turkish DATE-Q demonstrated moderate criterion validity with the DKT2 (r = 0.576, P < .001). Internal consistency was excellent (Cronbach’s alpha = 0.90), and test–retest reliability was strong (ICC = 0.80-0.96). Conclusion: The Turkish version of the DATE-Q is a moderately valid and strongly reliable tool for assessing diabetes knowledge. Its use in research and clinical settings can support endocrinologists, physiotherapists, dietitians, and nurses in identifying patients’ knowledge gaps, developing tailored education programs, and promoting effective self-management strategies. Incorporating the DATE-Q into multidisciplinary diabetes care may enhance patient outcomes and help prevent diabetes-related complications. © 2026, AVES. All rights reserved.
Disaster management in Türkiye: a qualitative study on healthcare professionals
(2026) Mutlu, Hatice; Türkoğlu, Mesut Can; Erküvün, Kübra Sezen
Background Disasters pose complex challenges that extend beyond immediate emergency response, requiring well-coordinated, resilient, and health-centered disaster management systems. In disaster-prone countries such as Türkiye, understanding how health services, particularly specialized medical rescue teams, function in real disaster settings is critical for identifying systemic gaps and improving preparedness, coordination, and response capacity. The aim of this study is to examine the managerial and organizational challenges encountered in disaster management processes based on the experiences of UMKE personnel who have served during various disasters in Türkiye, and to derive implications for strengthening disaster preparedness and response capacity. Methods The study was conducted using a qualitative research design. In-depth interviews were carried out with healthcare professionals who participated in disaster response. The data were analyzed using thematic analysis, and the findings were categorized into five main themes: shortages of logistics and equipment; working conditions and training; social awareness; lack of coordination, communication, and organization; and institutional structure and local empowerment. Results The findings revealed that road damages hindered transportation and that alternative transportation plans were inadequate. Disaster training were not fully adapted to field conditions. Public disaster literacy was found to be low. Insufficient coordination and communication disrupted interventions. In addition, the study identified psychosocial support, community-based education, and institutional capacity building as critical components of effective disaster management. Conclusions This study was designed to capture the personal perspectives of healthcare professionals who directly confronted disasters and gained first-hand experience of related processes. Therefore, it provides a unique contribution by presenting a disaster management framework through the lens of healthcare professionals and by highlighting areas in need of improvement for policymakers in this field.
Comparison of color stability and wear resistance of different 3D printed definitive restorative resins
(2026) Gündoğan, Zülal Çoban; Özyılmaz, Özgün Yılmaz; Kara, Özlem
Statement of problem: Subtractive manufacturing in computer-aided design and computer-aided manufacturing (CAD-CAM) systems has been extensively studied, but additive manufacturing materials, particularly those used for definitive restorations, are relatively new and data regarding their clinical performance remain limited. Purpose: This in vitro study aimed to evaluate the color stability of 3 different 3-dimensionally (3D) printed definitive restorative materials under 5 different Commission Internationale de l'Eclairage illuminants and to compare their metameric color differences. In addition, the mechanical wear resistance of these materials was evaluated independently. Material and methods: A total of 135 specimens with thicknesses of 1.0, 1.5, and 2.0 mm were 3D printed according to the manufacturers' protocols. Color was evaluated before and after thermocycling under 5 illumination conditions (D65, D50, A, F11, F2). ΔE00 values were analyzed using mixed-design analysis of variance (α=.05). Wear was evaluated in 2.0-mm specimens using simulated mastication and volumetric analysis, and the wear data were analyzed using the Kruskal-Wallis test (α=.05) RESULTS: All specimens exposed to illuminant A exhibited clinically unacceptable color changes, regardless of material type or thickness. Under illuminants D50, F11, and F2, color changes were perceptible but remained within clinically acceptable limits. Thermal aging significantly reduced ΔE00 values (P=.001). Although wear was observed in all groups, no significant differences were found in volumetric loss (P=.139). Conclusions: Illuminants significantly influenced color stability across all resin types and thicknesses, whereas increased restoration thickness was associated with reduced color change perceptibility. In addition, no significant differences in wear volume were found among the tested 3D-printed definitive restorative materials.
