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Öğe Stress distribution in titanium and zirconia implants with full-arch prostheses: a finite element analysis(2026) Saz, Bahattin; Özyılmaz, Özgün Yusuf; Özyılmaz, Özge DoğanayObjective: The stress distribution and mechanical properties of materials used in dental implants are critically important for implant success and long-term stability. The aim of this study was to evaluate the stress distribution on implant, prosthetic system, and peri-implant tissues when titanium and zirconia implants are used with different framework materials using three-dimensional finite element analysis (FEA). Methods: A three-dimensional edentulous maxilla model was reconstructed from computed tomography data, and twelve finite element models were subsequently developed based on this anatomy to represent different implant and framework configurations. Within the scope of the study, titanium and zirconia implants were modeled in combination with cantilevered and noncantilevered prosthesis types and Co-Cr, PEKK, and zirconium framework materials. An oblique static load of 200 N was applied at a 45° angle in the palato-buccal direction to the central fossa of the first molar on the models. The Von Mises and principal stress distributions in the implant, abutment, screw, framework, and peri-implant bone were examined. Results: The highest Von Mises value was recorded in the cantilevered PEKK framework zirconia implant model (378.388 MPa). Compared with the Co-Cr and zirconium framework, the PEKK framework resulted in higher Von Mises stresses and greater prosthesis displacement. The presence of a cantilever increased stress levels in all framework materials, and stress concentrations were observed particularly in posterior abutments and peri-implant bone. Furthermore, stress values in trabecular bone were recorded above the physiological threshold level in the cantilevered PEKK framework models. Conclusions: Although zirconia implants exhibited greater stress than titanium implants, they remained within safe limits. Rigid framework materials and cantilever-free designs provide more balanced biomechanical results.Öğe The Role of Boron in the Diet and as a Dietary Supplement(2026) Kuru Yaşar, Hülya; Yarat, AyşenBoron is a naturally occurring trace element that has historically received limited attention in human nutrition and is of increasing biological importance. It is widely found in nature, primarily through food and drinking water, and has attracted attention for its potential health benefits. Emerging evidence suggests that boron supplementation may support bone health, cognitive function, mineral and hormonal metabolism, the antioxidant defense system, body weight regulation, and inflammation reduction. This review examines the physiological roles, health, and clinical effects of boron, both through dietary intake and supplementation. Although not currently classified as an essential nutrient, boron appears to be biologically important, and it is thought that supplementation may have positive effects on many health conditions, including osteoporosis, osteoarthritis, body weight regulation, cancer prevention and treatment, diseases associated with oxidative stress, neurological and mental disorders, and hormonal imbalance.Öğe Spatial characterization of the phoenixin receptor in the lacrimal and major salivary glands(2026) Gürler, Esra Bihter; Elmas, M.A.; Eyüpoğlu, I.P.; Arbak, S.; Çevik Aras, H.Objective: To identify and characterize the expression of phoenixin receptor GPR173 (G protein-coupled receptor 173) in the rat lacrimal and major salivary glands (parotid, submandibular, and sublingual). GPR173 is associated with hormonal regulation and autoimmunity; however, its expression in exocrine glands has yet to be characterized. Design: Under anesthesia, eight Sprague Dawley rats underwent bilateral dissection of the lacrimal and major salivary glands. GPR173 expression was assessed using Western blotting and immunofluorescence. Immunoreactivity was quantified using ImageJ software and compared using the Friedman test with DunnBonferroni post hoc analysis. Results: GPR173 protein expression was found in all examined exocrine glands. In the major salivary glands, GPR173 was detected in both acinar and ductal cells, while expression was confined to acinar cells in the lacrimal gland. The submandibular and parotid glands showed significantly higher immunoreactivity than the lacrimal gland (p < .01 and p < .05, respectively). Ductal epithelium showed stronger immunopositivity compared to acinar epithelium. GPR173 immunoreactivity was also observed in periductal stromal tissue in major salivary glands. Conclusion: GPR173 expression was observed in both acinar and ductal cells of all major salivary glands, while in the lacrimal gland, it was restricted to the acinar cells. This differential localization suggests tissue-specific relevance.Öğe Assessment of Surface Roughness and Bacterial Adhesion of Occlusal Splints Fabricated with Different Layer Thicknesses, Polishing Techniques and Build Orientations(Multidisciplinary Digital Publishing Institute (MDPI), 2026) Dede, Merve; Saygılı, Sina; Topçuoğlu, NursenThis study evaluated the combined effects of build orientation, layer thickness, and polishing protocols on surface roughness and bacterial adhesion of occlusal splints. Ten disc-shaped specimens (Ø16 × 3 mm) were fabricated for each group using a digital light processing (DLP)-based 3D printer. Specimens were printed at two orientations (0° and 90°) and two layer thicknesses (50 and 100 µm) using a splint resin. Surface roughness was measured with a contact profilometer, and bacterial adhesion was measured by optical density (OD) readouts for Streptococcus mutans using a spectrophotometer. Surface morphology was examined by field-emission scanning electron microscopy (SEM). Statistical analyses were performed using jamovi. Because normality and/or homogeneity assumptions were not met, robust analysis of variance was applied. Polishing protocol significantly affected surface roughness (Ra) values. Unpolished specimens showed the highest Ra values, whereas mechanical polishing combined with centrifugation produced the lowest values. No significant main effects of polishing protocol, layer thickness or orientation were observed for bacterial adhesion. SEM findings supported the roughness results. Surface roughness was primarily influenced by polishing protocols and their interactions, whereas bacterial adhesion remained relatively stable. The weak Ra–OD correlation indicated that surface roughness alone was not a reliable predictor of bacterial adhesion.Öğe Postoperative thirst in children: Experience of children's, parents and health professionals(2026) Şensoy, Özlem; Mert, Sabiha; Aydın, Ayfer; Şahin, CeylanPurpose: Thirst is one of the most distressing symptoms experienced by pediatric patients after surgery. This study aimed to explore and document the experiences of children, parents, and health professionals regarding postoperative thirst, providing insights to develop care strategies that address the emotional and psychological needs of pediatric patients. Design: A qualitative approach was employed to gather in-depth insights into the experiences of children, parents, and health professionals regarding postoperative thirst. Methods: Purposive sampling was employed in a Pediatric Surgery Unit, involving a total of 40 participants: ten children, sixteen parents, and fourteen health professionals. Semi-structured interviews were conducted with all participants, and the transcribed data were analyzed using qualitative content analysis. Findings: The content analysis revealed five themes: physical symptoms, feeling of discomfort, lack of knowledge, coping strategies, and suggestions. Conclusions: This qualitative study highlights the critical importance of addressing postoperative thirst in enhance the physical and emotional well-being of pediatric patients, emphasizing that thirst is not merely a physical symptom but a multidimensional experience that significantly affects children and their families. Implications to practice: This findings supports health professional should be educated on recognizing and managing postoperative thirst as a symptom in postoperative care. Integrating evidence-based thirst management strategies into postoperative care protocols can significantly improve patient outcomes, reduce anxiety, and promote recovery, ultimately advancing the quality of pediatric surgical care. © 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.Öğe The Impact of Social Media Based Advice on Health Behaviors in Digital Health Communication(Journal of Health Communication, 2026) Öngel, Volkan; Yurttaş, Özge Uluğ; Ateş, Gözde Bozkurt; Gümüşboğa, Yağmur; Mutlu, HaticeThe growing visibility of social media influencers has increased interest in their role in shaping health beliefs and behaviors. While prior research has largely focused on message characteristics or direct behavioral outcomes, less attention has been given to the conditions under which influencer-based health messages are interpreted and translated into action. This study conceptualizes influencer-based health communication not as a direct persuasive force, but as a conditional communication process. Drawing on Uses and Gratifications Theory and the Health Belief Model as interpretive frameworks, it examines how individuals engage with health-related influencer content, how such engagement relates to health perceptions, and how digital health literacy shapes message interpretation.Survey data from social media users were analyzed using Firth's penalized logistic regression to address the rarity of influencer-driven health behavior. Findings indicate that, despite high levels of exposure to influencer-based health content, the translation of such exposure into health-related action remains limited. Exposure to health content significantly increases the likelihood of influencer-driven behavior, whereas higher digital health literacy reduces this likelihood, suggesting a protective effect. Additionally, perceived commercial intent decreases the probability that influencer recommendations lead to action.By distinguishing between engagement and behavioral change, this study reframes influencer effects as conditional outcomes shaped by motivations, perceptions, and interpretive competencies. It contributes to health communication literature by moving beyond linear persuasion models and highlighting the critical role of digital health literacy. The findings also offer practical implications for strengthening individuals' ability to critically evaluate health information in digital environments.Öğe Post-transcriptional knockdown of BRCC3 via siRNA-loaded niosomes modulates autophagy and endoplasmic reticulum stress in rotenone-induced Parkinson's Disease model(2026) Gürel, Çevik; Tut, Ezgi; Kuşçu, Gökçe Ceren; Buhur, Aylin; Germiyan, Özgün Selim; Leila Sabour-Takanlou; Maryam Sabour-Takanlou; Güler, Cem; Yavaşoğlu, Nefise Ülkü Karabay; Oltulu, Fatih; Yavaşoğlu, AltuğParkinson's Disease (PD) is a prevalent neurodegenerative disorder. Recent studies implicate BRCA1-/BRCA2-containing complex 3 (BRCC3) in PD-related mechanisms such as ubiquitin-proteasome system dysfunction. This study aimed to evaluate the therapeutic potential of BRCC3 silencing via systemically administered siRNA-loaded niosomes in a rotenone-induced rat model of PD. Niosomes were synthesised by thin-film hydration, and three BRCC3-targeted siRNA sequences were tested in primary midbrain dopaminergic neurons. The most effective sequence, identified by Real-Time Quantitative PCR (RT-qPCR) and immunofluorescence, was used for in vivo studies. The PD model was induced in adult male rats (n = 24/group) by subcutaneous rotenone administration (2 mg/kg/day) for 35 days. In the in vivo phase of the study, behavioural, biochemical, in vivo imaging (IVIS), histological, and RT-qPCR analyses were performed. IVIS analysis confirmed brain accumulation of niosome-siRNA complexes within 3-5 h. Complementary analyses demonstrated that siRNA treatment significantly enhanced locomotor performance, restored redox homeostasis and dopamine levels, attenuated neuronal loss, upregulated autophagy-related proteins (↑LC3-II, ↑Beclin), suppressed endoplasmic reticulum stress markers (↓GRP78/Bip, ↓CHOP), elevated tyrosine hydroxylase expression, and reduced α-synuclein accumulation. In conclusion, siRNA-mediated suppression of BRCC3 via siRNA-loaded niosomes provides neuroprotection by modulating autophagy, ER stress, and antioxidant pathways, supporting BRCC3 as a promising therapeutic target for PD.Öğe Disaster management in Türkiye: a qualitative study on healthcare professionals(2026) Mutlu, Hatice; Türkoğlu, Mesut Can; Erküvün, Kübra SezenBackground Disasters pose complex challenges that extend beyond immediate emergency response, requiring well-coordinated, resilient, and health-centered disaster management systems. In disaster-prone countries such as Türkiye, understanding how health services, particularly specialized medical rescue teams, function in real disaster settings is critical for identifying systemic gaps and improving preparedness, coordination, and response capacity. The aim of this study is to examine the managerial and organizational challenges encountered in disaster management processes based on the experiences of UMKE personnel who have served during various disasters in Türkiye, and to derive implications for strengthening disaster preparedness and response capacity. Methods The study was conducted using a qualitative research design. In-depth interviews were carried out with healthcare professionals who participated in disaster response. The data were analyzed using thematic analysis, and the findings were categorized into five main themes: shortages of logistics and equipment; working conditions and training; social awareness; lack of coordination, communication, and organization; and institutional structure and local empowerment. Results The findings revealed that road damages hindered transportation and that alternative transportation plans were inadequate. Disaster training were not fully adapted to field conditions. Public disaster literacy was found to be low. Insufficient coordination and communication disrupted interventions. In addition, the study identified psychosocial support, community-based education, and institutional capacity building as critical components of effective disaster management. Conclusions This study was designed to capture the personal perspectives of healthcare professionals who directly confronted disasters and gained first-hand experience of related processes. Therefore, it provides a unique contribution by presenting a disaster management framework through the lens of healthcare professionals and by highlighting areas in need of improvement for policymakers in this field.Öğe Comparison of color stability and wear resistance of different 3D printed definitive restorative resins(2026) Gündoğan, Zülal Çoban; Özyılmaz, Özgün Yılmaz; Kara, ÖzlemStatement of problem: Subtractive manufacturing in computer-aided design and computer-aided manufacturing (CAD-CAM) systems has been extensively studied, but additive manufacturing materials, particularly those used for definitive restorations, are relatively new and data regarding their clinical performance remain limited. Purpose: This in vitro study aimed to evaluate the color stability of 3 different 3-dimensionally (3D) printed definitive restorative materials under 5 different Commission Internationale de l'Eclairage illuminants and to compare their metameric color differences. In addition, the mechanical wear resistance of these materials was evaluated independently. Material and methods: A total of 135 specimens with thicknesses of 1.0, 1.5, and 2.0 mm were 3D printed according to the manufacturers' protocols. Color was evaluated before and after thermocycling under 5 illumination conditions (D65, D50, A, F11, F2). ΔE00 values were analyzed using mixed-design analysis of variance (α=.05). Wear was evaluated in 2.0-mm specimens using simulated mastication and volumetric analysis, and the wear data were analyzed using the Kruskal-Wallis test (α=.05) RESULTS: All specimens exposed to illuminant A exhibited clinically unacceptable color changes, regardless of material type or thickness. Under illuminants D50, F11, and F2, color changes were perceptible but remained within clinically acceptable limits. Thermal aging significantly reduced ΔE00 values (P=.001). Although wear was observed in all groups, no significant differences were found in volumetric loss (P=.139). Conclusions: Illuminants significantly influenced color stability across all resin types and thicknesses, whereas increased restoration thickness was associated with reduced color change perceptibility. In addition, no significant differences in wear volume were found among the tested 3D-printed definitive restorative materials.Öğe Endocrine and metabolism modulating effects of paracetamol: From in vitro signaling to in vivo metabolic reprogramming in male mice(Elsevier, 2026) Entezari, Bita; Bozdağ, Deniz; Buhur, Aylin; Sabuncuoğlu, Suna; Yavaşoğlu, Altuğ; Orhan, Hande GürerObesity is a major global health challenge associated with a cluster of comorbidities, including metabolic syndrome and type 2 diabetes, necessitating a deeper understanding of the environmental factors contributing to this epidemic. This study investigated the in vitro adipogenic/lipogenic potential of paracetamol and its in vivo endocrine and metabolic modulating effects following prenatal exposure. Using the 3T3-L1 preadipocyte model, cells were exposed to paracetamol at physiologically relevant concentrations. Results demonstrated that paracetamol promoted lipid accumulation and upregulated G3PDH activity. Furthermore, low concentrations significantly increased the protein expression of key adipogenic regulators (PPARγ, C/EBPα, LPL, and SREBP1), suggesting interference with transcriptional cascades governing adipogenesis and lipogenesis. To assess in vivo effects, pregnant CD1 mice were exposed to paracetamol at three human relevant doses (Cmax/10, Cmax, and Cmax×10). In male F1 offspring, prenatal exposure resulted in increased anogenital distance and a higher incidence of sperm morphological abnormalities, indicating reproductive developmental alterations despite unchanged circulating hormone levels. Metabolically, offspring exhibited dyslipidemia characterized by elevated serum triglycerides and total cholesterol. Although body weight and glucose tolerance remained unaffected, lipidomic profiling of epididymal adipose tissue revealed pronounced remodeling, including the accumulation of neutral lipids and altered membrane phospholipid composition. This was accompanied by the upregulation of the adipogenic genes Pparγ, Lpl, and Fasn in adipose tissue. Collectively, these findings suggest that paracetamol may act as an endocrine modulator and metabolic disruptor when exposed prenatally, inducing latent metabolic dysregulation that may predispose offspring to metabolic syndrome later in life, even in the absence of overt obesityÖğe Comparison of tensile bond strengths of different impression materials used with custom trays fabricated by 3D printing(2026) Topalan, Onur; Özyılmaz, Özgün YusufBackground: This study aimed to evaluate and compare the tensile bond strength between four different impression materials and custom trays fabricated using various photopolymer-based threedimensional (3D) printing materials and one conventional method. Additionally, the effect of three surface treatments on bond strength was investigated. Methods: A total of 288 custom tray specimens were fabricated using four different techniques: Stereolithography (SLA), liquid crystal display (LCD), digital light processing (DLP), and a conventional light-cured urethane dimethacrylate (UDMA)-based acrylic. Each tray type was subjected to three surface treatments: adhesive-only, perforated-only, and perforated + adhesive. Four elastomeric impression materials were tested: polyvinylsiloxane, condensation silicone, polyether, and vinylsiloxanether. A standardized CAD model was used for all trays. The impression materials were applied onto the tray surfaces, and tensile bond strength was tested using a universal testing machine. Each condition was tested with six replicates (n = 6). Data were recorded in Newtons and converted to megapascal (MPa) using a standardized bonding surface area of 825 mm². Three-way ANOVA and Tukey’s post-hoc tests were conducted (α=0.05). Results: All three independent variables - printing technique, surface treatment, and impression material - had a statistically significant effect on tensile bond strength (p < 0.001). The highest mean bond strength (0.272 MPa) was observed with the DLP-printed tray using perforatedonly and polyether impression material. The lowest value (0.018 MPa) was found with the same tray type and surface treatment, but with condensation silicone. Among surface treatments, adhesive-only and adhesive + perforated trays showed significantly higher bond strengths compared to perforated-only groups (p = 0.001). Polyether showed significantly higher bond strength than all other impression materials, while condensation silicone yielded the lowest. Conclusions: Within the limitations of this in vitro study, tray fabrication method and surface treatment significantly influenced bonding with impression materials. The present findings may provide useful preliminary data for future clinical investigations evaluating retention characteristics of 3D-printed custom trays.Öğe Volumetric Three-Dimensional Evaluation of the Pharyngeal Airway After Orthognathic Surgery in Patients with Skeletal Class III Malocclusion(MDPI, 2025) Öz, Aslıhan Zeynep; El, Hakan; Öz, Abdullah Alper; Palemo, Juan MartinBackground: Orthognathic surgery significantly alters the dimensions of the pharyngeal airway. This study’s objective was to assess alterations in the pharyngeal airway volume via cone-beam computed tomography (CBCT) after orthognathic surgery in patients with skeletal Class III malocclusion. Methods: This retrospective study analyzed CBCT images from 23 patients with skeletal Class III malocclusion (13 females, 10 males), who were categorized into two groups based on the surgical approach: double-jaw and single-jaw surgery. The double-jaw group included 13 patients who underwent bilateral sagittal split osteotomy (BSSO) and Le Fort I osteotomy, whereas the single-jaw group included of 10 patients who had underwent BSSO only. CBCT images were evaluated both before surgery and at a minimum of three months after surgery. The oropharyngeal volume (OP), nasopharyngeal volume (NP), total airway volume, posterior airway space (PAS), and the most constricted area at the base of the tongue (minAx) were measured. Statistical analyses were performed using either paired t-tests or Wilcoxon signed-rank tests depending on data normality, with a significance level set at p < 0.01. Results: In the double-jaw group, a significant volumetric increase was observed in the nasopharynx (5316 ± 1948 mm3 to 6064 ± 1899 mm3 ; p = 0.010) and oropharyngeal volume decreased from 17,097 ± 5675 mm3 to 14,290 ± 5835 mm3 ; however, this reduction was not statistically significant (p = 0.017). In contrast, the single-jaw group showed a significant reduction in oropharyngeal volume from 15,620 ± 5040 mm3 to 12,444 ± 4701 mm3 (p = 0.010), with no significant change in nasopharyngeal volume (p = 0.551). Total airway volume significantly decreased only in the single-jaw group (from 20,452 ± 7754 mm3 to 16,846 ± 6529 mm3 , p = 0.010). Additionally, both groups exhibited marked decreases in PAS and minimum axial area values (all p < 0.01). Conclusions: Orthognathic surgery led to a significant volumetric increase in the nasopharynx in the double-jaw group, whereas the oropharynx volume significantly decreased only in the single-jaw group. Additionally, both surgical approaches resulted in a marked reduction in PAS and minimum axial area values, highlighting a notable impact on posterior airway dimensions.Öğe Color matching and metameric behavior of composite resins applied on anodized titanium surfaces: an in vitro spectrophotometric study(2026) Davut, Umur; Özyılmaz, Özge Doğanay; Özyılmaz, Özgün YusufBackground The aim of this study was to evaluate the color matching and metameric behavior of two different composite resins applied to titanium surfaces anodized to yellow and pink colors. The study aimed to determine the effects of anodization color and composite resin type on color performance in hybrid prosthesis and screw access hole closure applications. Materials and methods A total of 72 Grade 5 titanium discs (10 mm diameter × 2 mm thickness) were allocated to three anodization conditions: nonanodized/gray (0 V, n = 24), yellow (55 V, n = 24), and pink (80 V, n = 24). Each anodization group was subdivided into two subgroups: 3 M Filtek Z250 (microhybrid) and Tokuyama Estelite Sigma Quick (supra-nano hybrid) composite resins (A2 shade, 2 mm thickness; n = 12 per subgroup). Gray titanium + composite specimens were used as the reference for ΔE₀₀ calculations; yellow and pink anodization groups constituted the experimental groups. Color measurements were performed using a spectrophotometer (Konica Minolta CM-3700 A) under four light sources (D65, D50, incandescent A, and F11). Data were analyzed using mixed-design repeated-measures ANOVA, Bonferroni post hoc test, and independent samples t-test (α = 0.05). Results Anodization color (η² = 0.941) and composite type (η² = 0.952) demonstrated strong effects on ΔE₀₀ values (both p < 0.001). Light source showed statistically significant but clinically negligible effect (η² = 0.521, mean difference < 0.1 ΔE₀₀). Pink anodization exhibited higher color differences than yellow (2.84–2.99 vs. 1.53–1.62), and Tokuyama composite higher than 3 M (2.89–3.12 vs. 1.41–1.54) (both p < 0.001). Yellow+3 M combination showed lowest values (0.91–0.97), while Pink+Tokuyama showed highest (3.68–3.97). Metamerism index remained minimal (MI < 0.21). Conclusions Careful material selection is essential for optimal esthetics in anodized titanium-composite systems. Yellow anodization combined with a microhybrid composite demonstrated the most favorable color matching performance underÖğe Does knee joint proprioception differ according to age and gender in healthy adults?(2026) Kürklü, Galip Bilen; Yapalı, Gökmen; Arslan, Serdar; Çankaya, MusaBackground: This study aimed to investigate the effects of age and gender on knee joint position sense (KJPS) and movement sense (KJMS) in healthy adults. Methods: General physical activity was evaluated using the International Physical Activity Questionnaire-Short Form (IPAQ-Sf) and the Tegner Activity Scale (TAS). Knee discomfort was measured with the Lysholm Knee Score (LKS), and knee-related symptoms and functional status were assessed using the Knee Osteoarthritis Outcome Score (KOOS). Knee joint position sense (KJPS) was tested at 15°, 30°, 45°, and 60°, while knee joint movement sense (KJMS) was evaluated at 60° and 90°. Participants were categorized by gender (male, female) and age (18-29, 30-44, ≥ 45 years). Mixed-effects models were applied to analyze numerical data, and post hoc comparisons were conducted using least-squares means with Tukey's correction when appropriate. Results: The mean scores were KOOS = 96.41 ± 4.79, LKS = 96.41 ± 6.94, and IPAQ-Sf = 1772.02 ± 1332.10. Absolute errors for KJPS angles ranged from 2.58 to 3.42 between genders. KJMS at 60°-90° was measured between 1.82 and 1.95 s in males, and between 1.66 and 1.80 s in females. Significant differences in KJMS (60°-90°) were observed across age groups (F = 14.841, p < 0.001) and position-sense angles (F = 19.645, p < 0.001). Conclusion: KJPS assessment revealed significant differences in absolute errors by age and gender. Males demonstrated lower errors, while participants aged ≥ 45 years exhibited greater deviations than younger groups. No gender differences were identified in KJMS. Overall, proprioception declined significantly in the 45+ age group.Öğe Mitochondrial Dynamics‑Related Gene Regulation by Epigenetic Suppression of GCN5 Exerts Neuroprotective Effects in Rotenone‑Induced Parkinson’s Disease Model(2026) Kuşçu, Gökçe Ceren; Tut, Ezgi; Gürel, Çevik; Buhur, Aylin; Germiyan, Özgün Selim; Avcı, Çığır Biray; Güler, Cem; Şancı, Ebru; Yavaşoğlu, Nefise Ülkü Karabay; Yavaşoğlu, AltuğParkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by dopaminergic neuron loss and mitochondrial dysfunction. Recent studies implicate the histone acetyltransferase GCN5 in regulating mitochondrial homeostasis and oxidative stress. This study investigated the therapeutic potential of GCN5 silencing via systemically administered siRNA-loaded niosomes in a rotenone-induced rat model of PD. Niosomes were prepared using the thin-film hydration method, and the most effective siRNA sequence was selected through real time quantitative PCR (RT-qPCR) and immunofluorescence in primary mesencephalic neurons. Adult male rats were divided into four groups (n=24/group), and PD was induced with rotenone (2 mg/kg/day, s.c., for 35 days). Behavioral assessments, biochemical analyses, IVIS imaging, histopathology, immunohistochemistry, and RT-qPCR were conducted. IVIS confirmed brain accumulation of siRNA– niosomes within 3–5 h post-injection. GCN5 siRNA treatment significantly improved locomotor activity (p<0.05), decreased MDA levels (p<0.05), and restored SOD and dopamine levels (p<0.05). Molecular findings showed decreased GCN5 and mitochondrial fission-related gene Drp-1 expression, increased expression of mitophagy and biogenesis markers (↑Parkin, ↑PINK1, ↑Mfn2, ↑PGC-1α), elevated TH expression, and reduced α-synuclein accumulation. Histological analysis revealed preserved midbrain cytoarchitecture and reduced neuronal damage. In conclusion, these findings highlight that epigenetic silencing of GCN5 via siRNA-loaded niosomal delivery provides neuroprotection in PD by modulating the expression of genes involved in mitochondrial dynamics, offering preclinical support for its development as a novel therapeutic strategy.Öğe The relationship of the nutritional literacy level of individuals with diabetes on nutrition, quality of life, and metabolic control(2025) Seçkiner, Selda; Palamutoğlu, Merve İnce; Baş, Dilşat; İdiz, Cemile; Baş, MuratBackground Nutritional literacy is essential to improve the quality of life and metabolic control of individuals with diabetes. The aim is to evaluate the relationship between the Nutrition Literacy Level of Diabetic Individuals on Nutrition, Quality of Life, and Metabolic Control. Methods This study evaluated the relationship between the nutritional literacy level of 179 individuals diagnosed with diabetes aged 18-65 years with nutrition, quality of life, and metabolic control. The data of the study were obtained through a 5-part faceto-face survey method. Self-Perceived Food Literacy Scale (SPFLS) was used to measure the nutritional literacy of the participants, and the Quality-of-Life Short Form 36 (SF-36) was used to assess the health-related quality of life. Results Of the participants, 55.3% (n=99) had type-2 diabetes, and 44.7% (n=80) had type-1 diabetes. Their mean BMI was 27.83 ± 6.38 kg/m2 and an HbA1c value of 7.88 ± 2.13%. The mean age of individuals with type-1 diabetes participating in the study was 36.69±13.77, and those with type-2 diabetes as 55.60±9.91. In addition, when evaluated in terms of diabetes duration, the mean age was 13.17±9.74 years in those with type-1 diabetes and 11.80±8.13 years in those with type-2 diabetes. A statistically significant difference (p<0.05; p<0.001) was found in the SPFLS subfactor scores for ‘Social and Conscious Eating’ and ‘Food Label Analysis’ among diabetic individuals based on their diabetes type. However, no statistically significant difference was found in the SF-36 sub-factor scores (p>0.05). The study found a weak negative correlation between the BMI of individuals with type 1 diabetes and the SPFLS sub-factors ‘Resilience and Resistance’ and ‘Examining Food Labels’(p<0.05). In type-2 diabetics, BMI showed a weak positive correlation with ‘Food Preparation Skills’ and a weak negative correlation with ‘Resilience and Resistance,’ ‘Examining Food Labels,’ and ‘Physical Function’ (p<0.05). Additionally, in type-2 diabetics, BMI was weakly positively correlated with ‘Healthy Food Stockpiling,’ while the SF-36 sub-factors ‘Physical Function,’ ‘Role Physical,’ and ‘Vitality’ showed weak negative correlations” (p<0.05). While no significant statistical difference was found between the SPFLS sub-factors and HbA1c values in type-1 diabetic individuals (p>0.05), only the "Healthy Food Availability" sub-factor and HbA1c were found to be statistically significant in type-2 diabetic individuals (p<0.05).The subscales of SPFLS and SF-36 show significant relationships with the physical activity level, nutritional habits, body functions, mental health, social life, and general quality of life of individuals with ACCEPTED MANUSCRIPT ARTICLE IN PRESS ARTICLE IN PRESS diabetes, comprehensively revealing the disease's physical, psychosocial, and lifestyle effects on the individual. These findings support the significant associations of BMI and HbA1c with various lifestyle and health factors. Conclusions This study found that in type-1 diabetics, age and diabetes duration were positively correlated with food literacy and quality of life, while no such correlations were found in type-2 diabetics. Increasing food literacy in diabetes management may be an important component that can positively influence metabolic control, improve physical and mental health, and give individuals the option to manage their health and improve quality of life and well-being.Öğe Accuracy of Polyvinyl Siloxane Ether and Other Impression Materials in Full-Arch Implant Rehabilitation with Varying Angulations: A Comparative in Vitro Study(2025) Çapuroğlu, Akanay; Kayahan, Zeynep Özkurt; Kazazoğlu, EnderThis study evaluated the accuracy of digital and conventional impressions using different materials in fully edentulous jaws with implants at varying angulations. Two master models were fabricated: one with four parallel and another with four implants placed according to the all-on-4 protocol. Impressions were obtained using three materials—polyvinyl siloxane (PVS), polyether (PE), and polyvinyl siloxane ether (PVSE)—and a digital scanner (TRIOS 4). Conventional impressions were cast, scanned with an extraoral scanner, and compared with the reference models via Geomagic Control X software. In the parallel model, digital impressions exhibited significantly greater deviation values than PE (p=0.016). In the angulated model, PE demonstrated significantly lower deviation values compared to PVSE (p=0.007) and digital impressions (p=0.016). Deviation values increased with implant angulation in all groups, except PVSE, which showed no statistically significant difference. Polyether provided the highest accuracy. Implant angulation adversely affected accuracy across most impression methodsÖğe An Investigation of Sleep Quality, Stress, and Healthy Eating Status Among Shift-Working Firefighters: A Descriptive Cross Sectional Study(2025) Schoinas, Ezgi Sakar; Anık, Sema; Erkul, CahitThe aim of this study was to assess perceived stress, sleep quality, and healthy eating attitudes among fire department personnel and to examine the relationships among these variables, working in various units of the department daily. Methods: This descriptive cross-sectional study included 188 participants. Data were collected using a sociodemographic form, the Bergen Shift Work Sleep Questionnaire, the Perceived Stress Scale (Short Form), and the Attitude Scale for Healthy Nutrition (ASHN). Results: The ASHN total score was 75.84 ± 10.03, indicating generally positive nutrition attitudes. The Bergen score was 62.94 ± 19.15, reflecting moderate sleep disturbance. The Perceived Stress Scale score was 11.50 ± 2.61, showing moderate stress. Weak negative correlations were found between ASHN and the other scales, while Bergen and stress scores were positively correlated. Conclusions: Overall, shift-based, stressful firefighting work negatively affected sleep and stress.Öğe Predictive Value of Complete Blood Count Parameters for Alzheimer's Disease in Relation to Periodontal Status(Bentham Science, 2025) Karaduran, Kübra; Aydoğdu, Ahmet; Gelisin. Özlem; Günpınar, SadiyeGiven the role of inflammation in the development of both Alzheimer's disease (AD) and periodontal disease, it is plausible that periodontal disease may influence the progression of AD. Complete blood count (CBC) parameters may also serve as predictive indicators for this condition. This study investigated the predictive value of CBC parameters on the progression of AD in patients with periodontal disease.Öğe Deep Learning in neuroimaging for neurodegenerative diseases: State-of-the art, Challenges, and Opportunities(Elsevier B.V., 2025) Akan, Taymaz; Alp, Sait; Ledbetter, Christina Raye; Tafti, Ahmad P.; Arevalo, Octavio; Bhuiyan, Mohammad Alfrad NobelNeuroimaging is commonly used to diagnose neurodegenerative diseases (NDDs), providing crucial insights into brain changes before clinical symptoms manifest. Deep learning (DL) for neuroimaging can improve early diagnosis and disease monitoring. Clinical implementation of DL faces challenges in accurately representing realworld data. Recent models, particularly those focused on diagnostic categorization, have achieved high accuracy, but their applicability to patients is limited. Conflicting inferences have been reported, with findings from small cohorts generalizing conclusions without considering inter-scanner, intra- and inter-site variations. A theoretically feasible method involves gathering a comprehensive dataset that encompasses all patient demographics, but this presents practical challenges including harmonization, data incompleteness, class imbalance, and substantial costs. Existing research has also mostly focused on common NDDs like Alzheimer's Disease (AD) and Parkinson's Disease (PD). This contribution expands the literature by looking at a wider range of NDDs, exploring the latest advancements in applying deep learning algorithms to neuroimaging analysis for the diagnosis and monitoring of NDDs, including AD, Frontotemporal Dementia (FTD), Lewy Body Dementia, PD, Huntington's Disease, Amyotrophic Lateral Sclerosis, and Multiple Sclerosis. We emphasize how these approaches are handling spatial/temporal information available in brain volume imaging data. We conclude by discussing the challenges associated with the use of voxel-based, patch-based, ROI-based, and slice-based approaches in brain volume imaging. These challenges are further compounded by issues such as inter-site and inter-scanner variability, class imbalances in medical datasets, and the scarcity of accurately annotated data, all of which impact the performance and generalizability of deep learning models.
