Comparative cell viability of dentin-bonding adhesive systems on human dental pulp stem cells: time-dependent analysis

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dc.authoridAyça Sarıalioğlu Güngör / 0000-0002-8779-2949
dc.contributor.authorKazak, Magrur
dc.contributor.authorGüngör Sarıalioğlu, Ayça
dc.contributor.authorOzman, Zeynep
dc.contributor.authorDönmez, Nazmiye
dc.date.accessioned2024-06-12T06:48:12Z
dc.date.available2024-06-12T06:48:12Z
dc.date.issued2024
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Klinik Bilimler Bölümü
dc.description.abstractBackground Restorative materials are in prolonged contact with living tissues such as oral mucosa, dentin, pulp, periodontal, and periapical tissues. Therefore, the potentially harmful effects of these materials and their components on oral tissues should be evaluated before clinical use. This study aimed to compare the cell viability of different adhesive systems (ASs) on human dental pulp stem cells (hDPSCs). Methods Three ASs that combining methacryloyloxydecyl dihydrogen phosphate (MDP) monomer with new hydrophilic amide monomers [Clearfil Universal Bond Quick(CUBQ), Kuraray Noritake], self-reinforcing 3D monomer [Bond Force II(BFII), Tokuyama)], and dual-cure property [Futurabond DC(FBDC), VOCO] were used. Three (n=3) samples were prepared for each group. Dental pulp stem cells were isolated from ten patients’ extracted third molar teeth. Samples were incubated in Dulbecco’s modified Eagle’s medium (DMEM) for 24 h (h), 72 h, and 7 days (d) to obtain extracts. For the control group, cells were cultured without DBA samples. Cell viability of ASs extracts was measured using a cell proliferation detection kit (WST-1, Roche). Statistical analysis was performed using two-way ANOVA and post-hoc (Duncan) tests (p<0.05). Results At 24 and 72 h statistically significant differences were determined between control and BFII, control and FBDC groups (p<0.05), while no differences between control and CUBQ groups (p>0.05). On the 7th d, statistically significant differences were found between the control and experimental groups (p<0.05), while no differences between experimental groups (p>0.05). A statistically significant difference was detected for the BFII group over the three-time interval (p<0.05). The lowest cell viability was observed for the FBDC group at 24 h, and the difference was statistically significant when compared with 72 h and 7th d (p<0.05). Conclusion All ASs showed different cell viability values at various exposure times. It should be taken into consideration that pH values, as well as the contents of ASs, have a significant effect on the cell viability.
dc.identifier.citationKazak, Magrur & Sarialioglu Gungor, Ayça & Ozman, Zeynep & Dönmez, Nazmiye. (2024). Comparative Cell Viability of Dentin-Bonding Adhesive Systems on Human Dental Pulp Stem Cells: Time-Dependent Analysis. 10.21203/rs.3.rs-4194675/v1.en_US
dc.identifier.doi10.1186/s12903-024-04438-9
dc.identifier.endpage8
dc.identifier.issn1472-6831
dc.identifier.issue663
dc.identifier.scopus2-s2.0-85195533658
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/20.500.12941/210
dc.identifier.volume24
dc.identifier.wosWOS:001244350300007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGüngör Sarıalioğlu, Ayça
dc.language.isoen
dc.publisherResearch Square Platform LLC
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHücre çoğalması (WST-1)
dc.subjectCell proliferation (WST-1)
dc.subjectHücre canlılığı
dc.subjectCell viability
dc.subjectSitotoksisite
dc.subjectCytotoxicity
dc.subjectDentin yapıştırma adeziv sistemi
dc.subjectDentin bonding adhesive system
dc.subjectİnsan diş hamuru kök hücresi
dc.subjectHuman dental pulp stem cell
dc.titleComparative cell viability of dentin-bonding adhesive systems on human dental pulp stem cells: time-dependent analysis
dc.typeArticle

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